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Track 7 Diagnostic pathology
SUB TRACK Diagnostic pathology, Necroscopy, radiography, urinalysis, microscopic, tissues, haematological, Immunoserological techniques, cellular Pathology, Maxillofacial Surgery, Medicine, diagnose diabetes normal tissues, microscopic, pathologist, astrocytic tumors, therapeutic, cells
Diagnostic pathology establishes the aetiology of disease based on morphologic and/or clinical pathology findings, history, clinical symptoms, and results of auxiliary tests. In all fields of pathology, including in naturally occurring illness as well as illness brought on by experimentation, it is crucial. Separating the effects of disease that develops on its own from those that are brought on by the experimental drug or test object is crucial when doing experimental investigations. When an illness or death occurs unexpectedly in a colony of laboratory animals or just before a study is finished, diagnostic pathology is crucial.
Toxicologic pathology will continue to play a crucial role in the diagnosis and prevention of spontaneous, chemically-induced disease in the veterinary diagnostic laboratory. It is important to not undervalue the value of using naturally occurring chemically caused disorders as models because frequently these cases provide vital knowledge to our understanding of the toxicological mechanisms of these and related chemical families. One instance of this was the discovery that acute nephrotoxicity in dogs and cats was caused by melamine and cyanuric acid found in pet food.
Diagnostic Techniques in Pathology
The various methods employed in pathology include necroscopy, radiography, urinalysis, microscopic tissue inspection, haematological assays, and anatomical pathology. Another method that uses isolated, cultured microorganisms and results interpretation is diagnostic microbiology. To evaluate antibody-antigen reactions, immunoserological procedures such complement fixation, fluorescent antibody precipitation, hemagglutination, and ELISA (Enzyme Linked Immuno Sorbent Assay) are used.
There are typically two forms of sectioning used in electron microscopy for diagnostic pathology: thick and thin. With a glass knife, thick portions are divided. They are used to identify the desired location, such as a glomerulus in a kidney biopsy, for further ultramicrotomy. They are 0.5-2 m thick. Toluidine blue O (2 g in 1 percent aqueous sodium borate) is used to stain the thick sections, which are then inspected under a light microscope. The area of interest in a larger piece of implanted tissue may be cut out and thinly sectioned subsequently.
A diagnostic test is one that determines a condition’s source or nature. It aids in diagnosis. An examination’s diagnostic test may be used to determine a disease’s identity or to pinpoint the source of symptoms. A diagnostic exam may be used to pinpoint particular strengths and shortcomings when employed for other purposes.
It is also possible to employ diagnostic tests to identify the root of a behaviour or feature. A kid who is having trouble reading, for instance, might go through diagnostic testing to find out if they have dyslexia or a visual impairment.
A diagnostic test is typically intended to pinpoint a certain condition or group of traits. For instance, a test to identify diabetes would check for physical signs of the disease (such as high glucose blood levels a specific time after ingesting a controlled amount of sugar). While the kind and extent of a test for hearing loss would be very different. Diagnostic tests can also be used to detect software bugs, learning or social difficulties, and equipment problems in addition to medical conditions.
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